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1-25 of 29 Business Insights

  • Application Note

    Rapid, High Throughput Analysis of Compounds in Urine

    Urine is the matrix of choice as it can be collected easily and in large volumes. The variations within the urine matrix can adversely impact chromatographic separation and LC-MS/MS signal. The present study demonstrates that a simple “dilute and shoot” method coupled with the high sensitivity QSight? 220 triple quadrupole mass spectrometer system eliminates many of the complexities of sample preparation without compromising quantitation quality.

  • Application Note

    Metanephrine and Normetanephrine Quantification by SPE-LC-MS/MS

    Measurement of metanephrines and normetanephrine in plasma is challenging due to the low physiological concentrations, their hydrophilic nature1, and time consuming traditional sample preparation. With use of the PerkinElmer QSight? 220 triple quadrupole mass spectrometer, LC-MS/MS analysis was performed using the recently advanced solid phase extraction (SPE) sorbent technology (“load, wash, elute”) method protocol. As a result low levels of ME and NOR are detectable in plasma with short sample preparation and LC run time. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NOR in plasma.

  • Application Brief

    Analysis of Water-Soluble Vitamins (B2, B3) using Quasar SPP HILIC Column

    In 1906, English biochemist Sir Frederick Gowland Hopkins also discovered that certain food factors were important to health. All the vitamins we recognise today were discovered during the early 20th century. There are two groups of vitamins, fat soluble and water soluble. Both types are regarded as essential for normal growth and our overall well being. Vitamin B2 (Riboflavin) and vitamin B3 (Niacinamide) are water soluble vitamins, naturally occurring in a number of food stuffs including milk and eggs. Both vitamins are used as dietary supplements and can help lower cholesterol, prevent migraines. Hydrophilic interaction liquid chromatography (HILIC), as first suggested by Alpert in 1990 provides an alternative approach to effectively separate small polar compounds, on polar stationary phases, which are not well retained under reversed phase conditions. Water is the strongest solvent in HILIC and as mobile phases typically contain low levels of water the resulting methods are ideally suited for MS applications. This application brief illustrates the efficient separation of vitamin B2 and B3 applying the HILIC mode of separation using the Quasar SPP HILIC column.

  • Application Note

    A Quantitative Determination of Methadone and its Metabolite (EDDP) in Dry Blood Spots Using LC-MSMS

    The present research study provides a much simplified approach whereby a highly sensitive LC-MS/MS triple quadrupole mass spectrometer is used to measure directly the methadone and EDDP concentrations through the dried blood spot (DBS) samples.

  • Application Note

    Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection

    In this application, we describe a technique for the monitoring of six cannabinoids, including THC and CBD, in hemp seed oil by HPLC with PDA detection. Figure 1 shows the chemical structures for the six cannabinoids. In recent years, scientific knowledge regarding the composition and health benefits of edible hemp products has significantly increased. Hemp seed oil has been promoted as a good source of nutritious omega-6 and omega-3 polyunsaturated acids, and may be a cleaner, more sustainable alternative to fish oil.

  • Application Note

    Cannabis Analysis: Potency Testing Identification and Quantification of THC and CBD by GC/FID and GC/MS

    This application note will concentrate on the potency identification and quantification of THC and CBD in cannabis by Gas Chromatography. Other application notes will cover potency by HPLC, pesticide analysis and residual solvent analyses. Analysis of cannabis has taken on new importance in light of legalized marijuana in several states of the USA. Cannabis contains several different components classed as cannabinoids. Primary cannabinoids of interest are tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN). Positive identification and quantification of the THC/CBD ratio is a primary objective in the analysis of cannabis. Cannabis is analyzed for several different purposes.

  • Application Note

    Determination of the Nitrofuran Metabolites in Seafood using Ultra High-Performance Liquid Chromatography - Tandem Mass Spectrometry

    Nitrofurans are a class of broad-spectrum antibiotics that are widely used to kill or slow down the growth of bacteria in the aquaculture industry. The use of nitrofurans and their metabolites has been restricted by a number of countries and organizations within the European Union, United States and China, due to their harmful side effects to human health, and a Minimum Required Performance Limit (MRPL) 0f 1.0 μg/kg has been set for food, animal and aquaculture products.

    LC/MS/MS methods have been used for the detection of nitrofuran metabolites in many studies owing to the high sensitivity, selectivity and specificity of the methods. However, nitrofuran metabolites demonstrate low ionization efficiency with the electrospray ion source used in LC/MS/MS systems. Therefore, the use of a derivatization step for nitrofuran metabolites before extraction and LC/MS/MS analysis is crucial to meet the low regulatory limits of 1.0 μg/kg in food samples. The main purpose of this work is to determine the detection limits for nitrofurans using QSight® 210 LC/MS/MS system.

  • Application Brief

    Robust Simvastatin Analysis Under High Aqueous Conditions

    Statins are a group of medicines that can help lower the level of low-density lipoprotein (LDL) cholesterol in the blood and help prevent cardiovascular disease. LDL cholesterol is often referred to as "bad cholesterol", and statins reduce its production in the liver by competitively inhibiting HMG CoA reductase, an enzyme involved in cholesterol synthesis. The origin of simvastatin can be found as far back as the 1950’s, when scientists at Merck were researching the biosynthesis of cholesterol. But it was the isolation of HMG CoA reductase by Japanese biochemist Akira Endo whose research into the relationship between fungi and cholesterol biosynthesis was the springboard for the development of the synthetic statin Simvastatin, marketed under the trade name of Zocor. This application brief describes the robust HPLC analysis of the polar compound Simvastatin using a gradient which reaches 100% aqueous conditions.

  • Application Brief

    Robust Atorvastatin Analysis Under High Aqueous LC Conditions Application Brief

    The origin of statins dates back to the mid 1970’s when the Japanese biochemist, Akira Endo, isolated a factor from the fungus Penicillium citrinum which he identified as a competitive inhibitor of 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMG-CoA reductase). This substance, which he named compactin or mevastatin, was the first statin to be administered to humans. Statins competitively inhibit HMG-CoA reductase, an enzyme involved in cholesterol synthesis, especially in the liver. Used to prevent cardiovascular disease and to lower cholesterol levels, atorvastatin is one of a number of synthetic statins routinely produced. It is now the most commonly prescribed statin medication. This application brief describes the robust HPLC analysis of the polar compound atorvastatin using a gradient which reaches 100% aqueous mobile phase conditions.

  • Application Note

    Analysis of Aflatoxins by UHPLC

    Aflatoxin B1 is considered to be the most genotoxic of the mycotoxins, and, when ingested by cows, is converted to aflatoxin M1 which has been shown to cause liver cancer in certain animals.

  • Application Note

    High Sensitivity Methotrexate Quantitation in Human Serum Utilizing

    This application note outlines a rapid LC-MS/MS research method utilizing the QSight? 220 triple quadrupole mass spectrometer. The developed method provides exceptional results for the quantitation of methotrexate in serum especially in terms of LLOQs and linearity.

  • Brochure

    Quasar Liquid Chromatography Columns Brochure

    Whether you’re testing food, water, or pharmaceuticals, success often depends on getting back to basics. So whatever your separation challenge, your choice of liquid chromatography (LC) column can make all the difference. Our Quasar portfolio of LC columns allows you to achieve rugged and reproducible results – batch to batch and column to column – with an all-encompassing, flexible solution that meets the diverse, changing needs of analysis. Ultrapure silica-based Quasar columns deliver a comprehensive range of chemistries, together with state-of-the art, optimized bonding technology to give you a versatile, high-performing analytical solution for your increasingly complex samples. For flexibility, we provide a wide range of column sizes, including shorter columns packed with smaller particle sizes for shorter run times and better productivity. Plus, our scalable columns facilitate easy method transfer between HPLC and UHPLCtechnology platforms – and the smaller particle sizes means optimized sensitivity for those applications. Whatever your separation need, we have a chemistry or dimension to fill it.

  • Application Brief

    HPLC Analysis of Tramadol Using SPP Column Technology

    Opiates, originally derived from the opium poppy, have been used for thousands of years for both recreational and medicinal purposes.

  • White Paper

    Advantages and Applications of Superficially Porous Particle Columns in Liquid Chromatography

    The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superficially porous particles has received considerable attention. This white paper gives an overview about the theory behind the success of superficially porous particles technology and presents a summary of its latest applications.

  • Flyer

    NexSAR HPLC-ICP-MS Speciation Solution

    Speciation studies can provide comprehensive information regarding ion mobility, bioavailability, and toxicity. Consequently, many modern labs are expanding their analytical portfolio to include HPLC-ICP-MS. For such studies, it is advantageous to have an intuitive, user-friendly system that meets the user requirements for automation, ultra-low baseline, powerful integration, and flexible data export. And no one offers a more integrated, versatile, and inert speciation solution than PerkinElmer - accelerate your speciation analysis with the NexSAR? HPLC-ICP-MS Speciation Solution.

  • Application Note

    Cannabinoid Monitoring in Dried Cannabis Flowers

    This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

  • Application Brief

    HPLC Analysis of Vitamin C Using Quasar Biphenyl Column

    Vitamin C, also known as ascorbic acid and L-ascorbic acid, is a water soluble vitamin found in various foods including peppers, kiwifruit, oranges and kale. It is regarded as an essential nutrient to prevent scurvy, involved in the repair of tissue and also thought to lower cancer risk. It is required for the functioning of several enzymes and is important for immune system function. Since it’s isolation in 1928 it was the first vitamin to be commercially produced. Today it is widely available as a dietary supplement. This application brief describes use of a Quasar biphenyl column in the analysis of vitamin C.

  • Application Brief

    HPLC Analysis of Plavix Using Quasar C8 Column

    The medication known as Plavix is an antiplatelet drug which prevents platelets in your blood from sticking together to form an unwanted blood clot, that could block an artery. It is used to lower the risk of having a stroke, or serious heart problem after you've had a heart attack, or aid with circulation problems as it helps to keep blood flowing smoothly in the body. The patent expired in 2012 and the drug is now also sold under many generic brands. Plavix tablets contain the active ingredient clopidogrel hydrogen sulphate, which is readily analysed by HPLC. This application brief describes use of a Quasar C8 column in the analysis of Plavix.

  • Application Brief

    Amoxicillin Analysis Using Quasar C18 HPLC Column

    Antibiotics are natural substances released by bacteria and fungi which are capable of killing, or inhibiting, competing microbial species. This phenomenon has long been known; but it was not until 1928 that penicillin, the first true antibiotic, was discovered by Alexander Fleming, Professor of Bacteriology at St. Mary's Hospital in London. However, it was 12 years later that penicillin was isolated and developed as a medicine by Howard Florey and Ernst Chain. Since this breakthrough in therapeutic medicine, derivates of penicillin were developed with increased efficacy levels. Amoxicillin is one of several such semisynthetic penicillin’s. First discovered in the 1960’s its patent has now expired. Consequently, amoxicillin and co-amoxiclav is now marketed under many different trade names worldwide. It is commonly used in the treatment of numerous infections including pneumonia, skin infections and Lyme disease. This application brief illustrates the rapid analysis of amoxicillin using the Quasar C18 column.

  • Application Brief

    Rapid LC Analysis of Cephalosporins Using Quasar Biphenyl Column

    The aerobic mold which yielded cephalosporin C was found in the sea near a sewage outfall nearby Cagliari harbour, Sardinia, by the Italian pharmacologist Giuseppe Brotzu in July 1945. Since their discovery and subsequent commercialization in 1964, the cephalosporins today are broad-spectrum ?-lactam antibiotics used for the treatment of a number of bacterial conditions including septicaemia, pneumonia and meningitis. The pharmacology of cephalosporins is similar to that of the penicillin class of compounds. This application brief describes use of a Quasar biphenyl column in the analysis of several cephalosporins, a mixture of first and second generation ?-lactam antibiotics.

  • Application Brief

    Rapid and Robust HPLC Analysis of Indoxyl Sulfate Using Quasar C18 Column

    Indoxyl sulfate is one of the most extensively studied solutes that accumulates in the plasma when the kidneys fail. Originally called "indican", it was first isolated by Obermayer and Popper in 1911. High concentrations of indoxyl sulfate in blood plasma are known to be associated with the development and progression of a number of pathological conditions including chronic kidney disease and vascular disease. The scientific literature documents the use of older generation C18 columns, using type A silicas. This application brief will illustrate the application of a new generation C18 column, based on type B silica, for the analysis of indoxyl sulfate, Figure 1, as part of a research study to measure the total levels in a simulated blood serum environment.

  • Application Brief

    Sulfa Drugs Analysis Using Quasar C18 HPLC Column

    Sulfa drugs are accredited as the first set of compounds to exhibit antibiotic properties used to prevent bacterial infections in humans. The first sulfonamide, trade named Prontosil, was developed by Bayer in 1932. The subsequent years saw rapid development of antibacterial drugs and by the 1940’s sulfanilamide was widely used. Though the medicine was relatively safe, allergic reactions such as skin rashes, fever and nausea were common place. With the introduction of less-toxic derivatives and especially with the mass production of penicillin, its use declined. These short acting synthetic sulfa drugs are effective against a wide range of pathogenic microorganisms and are now commonly used in veterinary medicine. Sulfathiazole administered to cattle in combination with penicillin and chlortectracyline has been shown to yield higher rates of weight gain and improved feed efficiency. This application brief illustrates the efficient separation of three sulfa drugs using the Quasar C18 HPLC column.

  • Application Brief

    Analysis of Naproxen using Quasar SPP C18 Column

    Naproxen is a nonsteroidal anti-inflammatory drugs (NSAIDs) used to treat certain types of arthritis and other acute inflammatory conditions. It was first released to the prescription drug market in 1976 under the name Naprosyn. Then a few years later its counterpart salt, naproxen sodium, was released for prescription use and is used predominately in formulations today. The HPLC analysis of Naproxen on a porous silica C18 column is well documented in the literature. However, with the current trend in liquid chromatography being towards higher kinetic efficiency and shorter analysis time the subsequent development in column technology has realised that, without the need to change instrumentation. This application brief will illustrate the application of a superficially porous particles (SPP) column for the analysis of naproxen.

  • Application Brief

    Steroid Analysis using Quasar AQ HPLC Column

    Prednisolone, Prednisone and Cortisone are commonly used steroids to treat a range of inflammatory conditions and autoimmune disorders. These synthetic derivatives of hydrocortisone were developed and approved for medicinal use as early as the 1940’s. American chemists first identified Cortisone as having a therapeutic benefit in the treatment of rheumatoid arthritis and it was commercialized by Merck in 1948. The first commercially feasible synthesis of prednisone was carried out in 1955 in the laboratories of the Schering Corporation. A C18 HPLC column can be used for the analysis of these synthetic steroids, but they are not well retained, and resolution is incomplete. This application brief will look at the differences in chromatography between the Quasar C18 and AQ phase chemistries for the analysis prednisolone, prednisone and cortisone.

  • Application Note

    Chromium Speciation in Drinking Water by LC-ICP-MS

    Knowing the concentration of hexavalent chromium in environmental systems and samples which will be consumed is more important than knowing the total chromium concentration. This is especially true for drinking water. This work describes the use of LC-ICP-MS to measure both trivalent and hexavalent chromium in drinking waters, with the goal of presenting a suitable methodology for analyzing the range of concentrations encompassed by the new legislation.

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