For research use only. Not for use in diagnostic procedures.
The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters.
Features and Benefits
|Imaging Modality||Optical Imaging|
|Optical Imaging Classification||Bioluminescence imaging, Fluorescence Imaging|
|Product Brand Name||IVIS|
Cerenkov Emission from radioisotopes in tissue,Optical imaging detects photons in the visible range of the electromagnetic,spectrum. PET and SPECT imaging instruments are sensitive to photons in the much,higher energy range of x-rays and gamma rays. While the PET and SPECT probes,which can generate Cerenkov light in tissue will continue to produce the relevant,gamma- and x-rays, visible photons will be produced from the Cerenkov emission,which the IVIS? will detect.,In beta decay emitters such as PET probes and isotopes such as 90Y, 177Lu, 131I and 32P,the beta particle will travel in the tissue until it either annihilates with an electron or,loses momentum due to viscous electromagnetic forces.,It is possible that the beta (electron or positron),is relativistic, traveling faster than the speed,of light in the tissue. While it is impossible,to travel than the speed of light in a vacuum,(c), the speed of light in tissue is v = c / n,where n is the tissue index of refraction and,n = 1. Cerenkov photons will be generated,by a relativistic charged particle in a dielectric medium such as tissue.
Pre-clinical in vivo imaging application note on Bioimaging of Gene Delivery with In Vivo-jetPEI?. The delivery of nucleic acids into cells is essential for basic research as well as medical applications such as gene therapy. Viral vectors are efficient carriers, but their use is limited because of their safety (induction of immune response, virus-associated pathogenicity). These concerns have increased the interest of non-viral methods for in vivo gene delivery. Second generation of non-viral vectors offers improved performance and safety, potentially providing an alternative to viral gene delivery.
With the potential to treat a wide range of disease, from organ damage to congenital defects, stem cell research and tissue engineering form the underlying basis of regenerative medicine. Significant advances in the science of skin regeneration, for example, have now made it possible to develop and grow artificial skin grafts in a lab for treatment of burn victims. Other therapeutic applications include the use of stem cells to treat and repair central nervous system diseases such as ischemia and cerebral palsy, cardiovascular diseases, as well as autoimmune diseases including type I diabetes.
IVIS Lumina Series III Integrating Gold Standard Bioluminescence and Fluorescence In Vivo Technologies. The IVIS Lumina Series III from PerkinElmer provides an expandable, sensitive imaging system that is easy to use for both fluorescent and bioluminescent imaging in vivo. The Lumina Series III platform offers a full spectrum of wavelengths to perform almost any in vivo optical application with a tunability of 20 nm. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. As the leading optical imaging platform for in vivo analysis, IVIS systems include a range of practical accessories developed through experience in research laboratories worldwide.
Adaptive Fluorescence Background Subtraction Pre-clinical in vivo imaging technical note for IVIS Imaging Systems. Instrument background occurs when excitation light leaks through the emission filter. This occurs more frequently when the excitation and emission filters are narrowly separated. The ring you see is a result of non specific light reflecting off of the stage at an incident angle and passing through the filter causing what appears as leakage around the edges.
Auto-exposure technical note for IVIS pre-clinical imaging systems
Subtracting Background ROI from a Sequence
Determine Saturation for IVIS imaging systems - technical note
Technical notes for Drawing ROIs for IVIS in vivo imaging systems. The circle, square, free draw, or grid (for well plates) can be used to draw your ROIs. ROI selections,are user-specific and are dependent on the model being analyzed. It is irrelevant which shape that is used for a particular ROI.
Not only is it possible to load multiple images as a group, for example multiple days of a longitudinal study, but it is also possible to load multiple images and Overlay them i.e. bioluminescent tumor with fluorescent targeted drug acquired in two separate images.
Acquiring the most accurate quantitation of your bioluminescent sources requires a close understanding of the underlying kinetics involved in producing and capturing the detected light. After injection, the substrate for your bioluminescent probe will di
For many studies, it may be desirable to open a group of images together, for example, analyzing multiple days of longitudinal study side by side using the same scale.
Subject ROI using IVIS imaging systems
Working with Image Math. Image Math is a rudimentary but effective method for Spectrum and Lumina users to subtract background from images without performing Spectral Unmixing.