PerkinElmer

吉林快三一定牛走势一:Drug Discovery Screening Reagents

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吉林快三走势图综合板 www.saanl.com

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE? TR-FRET, DELFIA? TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications,?protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA? – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire? Ultra? and AlphaScreen? SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen? – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own.?Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight? dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.?

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1523 Products & Services

  • SureFire principle

    AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

    The AlphaScreen? SureFire? p-STAT3 (p-Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • AlphaLISA immunoassay kits

    IL-6 (bovine) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for quantitative detection of bovine IL-6. This kit permits detection of bovine IL-6 in bovine serum, plasma, and cell culture supernatants.
    Price Range : 725.00 USD - 10700.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

    The AlphaScreen? SureFire? p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaLISA immunoassay kits

    IL-1β (bovine) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 672.00 USD - 10700.00 USD
  • IL-12 (human) AlphaLISA Detection Kit

    The AlphaLISA? immunoassay kit for human interleukin-12 (IL-12; p70) enables the quantitative determination of human IL-12 in buffer, serum, plasma, and cell culture media using a homogeneous AlphaLISA assay (no wash steps). This kit replaces part numbers AL239C and AL239F.
    Price Range : 575.00 USD - 10500.00 USD
  • AlphaLisa Analyte 2

    CYR61 (human), lyophilized (AlphaLISA)

    Human cysteine rich angiogenic inducer 61 (CYR61) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Cyr61 Detection Kit, but can also be ordered separately.
    List Price : 782.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-GSK-3β (Ser9) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-GSK-3β (Ser9) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaLISA immunoassay kits

    ApoA1 (human) AlphaLISA Detection Kit

    The AlphaLISA? immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
    Price Range : 644.00 USD - 19300.00 USD
  • Photo of Alpha CETSA 5,000 point kit

    NFκB p65 Alpha SureFire CETSA Target Engagement Assay Kit

    Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire? CETSA? target engagement assays. For use in combination with Pelago Bioscience's CETSA? (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to NFκB p65, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA? subscription from Pelago Biosciences is required for use.
    Price Range : 2432.00 USD - 7400.00 USD
  • AlphaLISA immunoassay kits

    IgG3 (mouse) isotyping AlphaLISA Detection Kit

    This AlphaLISA? immunoassay kit enables the isotyping of mouse IgG3 antibodies in cell culture media and non-mouse serum.
    Price Range : 725.00 USD - 10100.00 USD
  • AlphaLISA immunoassay kits

    IL-4 (bovine) AlphaLISA Detection Kit

    The AlphaLISA? immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 465.00 USD - 10200.00 USD
  • AlphaLISA immunoassay kits

    Testosterone AlphaLISA Detection Kit

    The AlphaLISA? Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • Hepatitis B Virus s Antigen (HBsAg) AlphaLISA Detection Kit

    The AlphaLISA? immunoDetection Kit for Hepatitis B Virus Surface antigen (HBsAg) enables the quantitative determination of HBsAg in cell culture media and buffer using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 15500.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaScreen Acceptor Beads

    AlphaScreen Unconjugated Acceptor Beads

    Unconjugated AlphaScreen? Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
    Price Range : 375.00 USD - 10500.00 USD
  • TDP-43 (human) AlphaLISA Detection Kit

    The AlphaLISA? immunoassay kit for human TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, cerebrospinal fluid (CSF), cell lysates, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 649.00 USD - 21100.00 USD
  • Photo of Alpha CETSA 5,000 point kit

    MEK1 Alpha SureFire CETSA Target Engagement Assay Kit

    Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire? CETSA? target engagement assays. For use in combination with Pelago Bioscience's CETSA? (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to MEK1, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA? subscription from Pelago Biosciences is required for use.
    Price Range : 2332.00 USD - 7100.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1512.00 USD - 6800.00 USD
  • AlphaLisa Analyte 2

    CA125 (human), lyophilized (AlphaLISA)

    Human Mucin-16 / Ovarian Carcinoma Antigen CA125 (CA125) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CA125 kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA immunoassay kits

    TNFα (porcine) AlphaLISA Detection Kit

    The AlphaLISA? immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
    Price Range : 649.00 USD - 21100.00 USD
  • AlphaLISA immunoassay kits

    IL-2 (human) AlphaLISA Biotin-Free Detection Kit

    AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 725.00 USD - 10500.00 USD
  • SureFire principle

    AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

    The AlphaScreen? SureFire? p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-NF-κB p65 (Ser536) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire? Ultra? Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-NF-κB p65 (Ser536) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra Total Akt1 Assay Kit

    The AlphaLISA? SureFire? Ultra? total Akt1 is a sandwich immunoassay for quantitative detection of Akt1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
    Price Range : 504.00 USD - 30400.00 USD
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